Human amnion-derived mesenchymal stem cells improved the reproductive function of age-related diminished ovarian reserve in mice through Ampk/FoxO3a signaling pathway

Abstract Background Age-related diminished ovarian reserve (AR-DOR) reduced the quality of oocytes, resulting in decreased female fertility. Aging is tightly related to abnormal distribution and function mitochondria, while mitophagy a major process maintain normal quantity mitochondria cells, especially oocytes which containing large number meet demand energy production during oocyte maturation subsequent embryonic development. Ampk/FoxO3a signaling crucial regulation mitophagy. It reported mesenchymal stem cells (MSCs) can improve function. Here we aim explore if human amnion-derived (hAMSCs) are effective improving AR-DOR mice whether involved. Methods The model were established by 32-week-old with 3–8 litters, significantly low serum sex hormone levels follicle counts. old divided into 5 treatment groups: saline (NS, control), 1% albumin (HSA, resolver), dose (LD, 5.0 × 10 6 cells/kg), middle (MD, 7.5 high (HD, 10.0 cells/kg). prepared hAMSCs injected through tail vein. Serum level, counts, fertilization rate, gestation little size, apoptosis granulosa stromal expression level Sod2, Ampk, ratio phosphorylated FoxO3a total ovaries examined. Results Our results show that after hAMSC transplantation, was improved, meanwhile repressed, Ampk both increased, increased Sod2 also observed. Conclusion demonstrate transplantation via tail-injection pathway.